Science - USA (2020-05-22)

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ACKNOWLEDGMENTS

We thank Q. Song (Lanzhou University) and X. Zhang (University

of Louisville) for advice on entophytic fungi and biochemical analysis

of trichothecenes, X. L. Zhang (Northeast Forestry University) for

providing some marker sequences of the Triticeae E genome,

and Y. Liang and J. Yu (Shandong Agricultural University) for

providing theFusariumstains.Funding: This work was supported

by the National Natural Science Foundation of China (31520103911,

31871610, and 31901492), the National Key Research and

Development Program (2016YFD0100102-2), the National Key

Program on Transgenic Research from the Ministry of Agriculture

of China (2016ZX08002003-002 and 2016ZX08009-003), and

the Agricultural Variety Improvement Project of Shandong Province

(2019LZGC016).Author contributions: H.W. and L.K. designed

the project. S.S.X., G.B., E.N., C.G., and H.O. supervised the project.

S.S., K.W., L.C., X.F., and F.N. performed bioinformatics analysis.

W.G., L.Z., B.H., Z.L., S.S.X., J.G., M.L., P.S., X.F.L., G.W., C.B., W.Z.,

X.C., J.W., L.D., W.C., W.L., G.X., J.Z., Y.H., Y.X., Y.G., W.J.L.,

Y.L., H.Y., J.L., X.L., Y.Z., and X.W. conducted experiments. X.M.

and A.L. performed the field work. H.W., S.S., G.B., and L.K. wrote

the paper with input from all authors.Competing interests:

The authors declare no competing interests. Patents with application

nos. 2020101464009 and 202010146399X are pending.Data and

materials availability:All data are available in the manuscript,

the supplementary materials, or at publicly accessible repositories.

These data in the public repositories include all raw reads and

assembled sequence data forTh. elongatumin NCBI under

BioProjectID PRJNA540081, the assembly and annotation

data ofTh. elongatum,andthedraftgenomesof7E/7D

substitution lines in the Genome Warehouse in BIG Data

Center under accession numbers GWHABKY00000000,

GWHABLF00000000, and GWHABLE00000000, which are

accessible athttps://bigd.big.ac.cn/gwh. All materials are

available from L. Kong upon request.

SUPPLEMENTARY MATERIALS

science.sciencemag.org/content/368/6493/eaba5435/suppl/DC1

Materials and Methods

Figs. S1 to S40

Tables S1 to S23

Captions for Data S1 to S3

References ( 36 – 93 )

MDAR Reproducibility Checklist

Data S1 to S3

View/request a protocol for this paper fromBio-protocol.

12 December 2019; accepted 26 March 2020

Published online 9 April 2020

10.1126/science.aba5435

Wanget al.,Science 368 , eaba5435 (2020) 22 May 2020 7of7

RESEARCH | RESEARCH ARTICLE