Extended Data Fig. 4 | Role of SCN-projecting ipRGCs in nonphotic
entrainment. a–c, Retinal innervation (CTB) (red) and NPY staining (green) in
the IGL and SCN in control and Opn4cre/+ Brn3bDTA / + mice. Representative coronal
sections are shown (a). NPY staining in the IGL (b) and SCN (c) were analysed in
three-month-old mice. Data are mean ± SEM (n = 5 mice for each genotype),
two-tailed Student’s t-test. d–h, Control and Opn4cre/+ Brn3bDTA / + mice were
exposed to TRF. Representative actograms are shown (d). The locomotor
activity before food access (e) and the food-anticipatory activity (f) were
measured. Data are mean ± s.e.m. (n = 7 control mice, 12 Opn4cre/+ Brn3bDTA / +
mice), by two-tailed Student’s t-test. A score analysis was performed for all
actograms obtained (g). Data are mean ± s.e.m. (n = 7 control mice,
18 Opn4cre/+ Brn3bDTA / + mice), by Student’s t non-parametric (Mann–Whitney)
test, two-tailed. Representative actograms obtained from Opn4cre/+ Brn3bDTA / +
mice under TRF (h). Scale bars, 100 μm (a, bottom), 200 μm (a, top).