Extended Data Fig. 6 | NPY signalling in the IGL–SCN circuit controls
nonphotic entrainment. a–d, Representative actograms obtained from
NPY-knockout (KO) (Npycre/cre) mice exposed to TRF are shown (a). The
locomotor activity 9 h before food access (b) and the food-anticipatory activity
(c) were measured for control and NPY-knockout mice. Data are mean ± s.e.m.
(n = 7 control mice, 11 NPY-knockout mice), two-tailed Student’s t-test.
Additionally, a score analysis was performed for all actograms obtained (d).
Data are mean ± s.e.m. (n = 7 control mice, 11 NPY-knockout mice), Student’s t
non-parametric (Mann–Whitney) test, two-tailed. In addition, a second mouse
line (Npytm1Rpa) was used to evaluate the effects of NPY ablation. Results
obtained from both NPY-knockout mouse lines were indistinguishable (data
not shown). e, The daily total amount of food consumed (during ad libitum
access to food and TRF) was measured in control, NPY-knockout (Npycre/cre), and
Npycre/+ mice bilaterally injected in the IGL with a control A AV (A AV5/
Syn-DIO-hChR2(H134R)-EGFP-WPRE-HGHpA) or A A encoding TenT (pA AV5/
CMV-DIO-eGFP-2A-TeNT). Under free-running conditions (constant darkness
and ad libitum access to food), the amount of food consumed by control and
NPY-knockout mice (P = 0.0267), as well as control and Npycre/+ mice (P = 0.008),
was significantly different. Data are mean ± s.e.m.(n = 5 mice for each
condition), two-way ANOVA, followed by Sidak’s multiple comparisons test. f,
g, Representative actograms obtained from Npycre/+ mice exposed to TRF and
injected with control A AV (f) or A AV encoding TenT (g) are shown.