Extended Data Fig. 8 | CRH neuron-mediated promotion of plasma cell
formation requires the splenic nerve. a, A schematic diagram of the
experimental setup. b, The gating strategy for NP-specific IgG+ SPPCs, from a
parental gate of B-lineage cells as in Fig. 1b. c, Batch-normalized NP-specific
percentage SPPC in relative units (R.U.). For each experiment, the average
SPPC% in the Sham–eYFP group is set as unit 1, against which percentage SPPC
in individual mice of the four groups in the same experiment was normalized to
obtain the R.U. for each animal. R.U. data are pooled from three independent
experiments. Each symbol denotes one mouse, and lines indicate means.
Two-sided unpaired t-test. YFP, A AV-DIO-eYFP group, 3Q, A AV-DIO-hM3D(Gq)
group.