Nature - USA (2020-05-14)

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nature research | reporting summary

October 2018

For a reference copy of the document with all sections, see nature.com/documents/nr-reporting-summary-flat.pdf

Life sciences study design

All studies must disclose on these points even when the disclosure is negative.

Sample size Two separate proteins were purified and formed a complex.

Data exclusions No data excluded.

Replication The crystal of the complex were obtained in duplicate. All attempts at replication were successful.

Randomization N/A

Blinding N/A

Reporting for specific materials, systems and methods

We require information from authors about some types of materials, experimental systems and methods used in many studies. Here, indicate whether each material,

system or method listed is relevant to your study. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response.

Materials & experimental systems

n/a Involved in the study

Antibodies

Eukaryotic cell lines

Palaeontology

Animals and other organisms

Human research participants

Clinical data

Methods

n/a Involved in the study

ChIP-seq

Flow cytometry

MRI-based neuroimaging

Eukaryotic cell lines

Policy information about cell lines

Cell line source(s) SF9 and Hi5 cells were bought from ATCC

Authentication SF9^ refered to website: https://www.atcc.org/products/all/CRL-1711.aspx

Hi5 refered to website

These two cell lines are all available in commercial company.

Mycoplasma contamination We confirm that all cell lines were negative for mycoplasma contamination

Commonly misidentified lines

(See ICLAC register)

No commonly misidentified cell lines were used