Extended Data Fig. 3 | Additional scRNA-seq analyses. a, Heat map showing
differential gene expression patterns of all cells in the 16 assigned cell clusters
(indicated at the bottom). Each column is one cell cluster, and each row
represents one gene. b, Heat map showing differential gene expression
patterns of two subclusters in cluster 11 (11-1 and 11-2). c, Expression levels
(as in the coloured expression scale) of 3 cluster-11 markers, Minicollagen 2,
Nematogalectin 1 and Nematogalectin 3, are shown in the t-SNE plots.
n = 797 cells. d–f, RNA ISH control with sense probe for Minicollagen 1 (d),
Nematogalectin 2 (e) and Collagen 6 (f). g, Expression levels of Xe_003623,
a non-conserved and uncharacterized cluster-2 and -12 marker gene, in each of
the 16 cell types defined by scRNA-seq. Violin plot (Methods) show the
distribution of gene expression in each of the 16 clusters. Cell numbers in cell
clusters 1–16 were 2,794; 2,704; 2,073; 1,679; 1, 511; 1,374; 1,248; 1,069; 986; 923;
797; 649; 575; 321; 246; and 185, respectively. h, i, RNA ISH of Xe_003623 (h) and
Astacin-like metalloendopeptidase 2 (i) using anti-sense and sense probes.
In d–f, h, i, more than 12 polyps from 3 independent experiments were used for
each probe. Scale bars, 100 μm.