Article
Extended Data Fig. 4 | Additional analyses for endosymbiotic cells. a, Violin
plots of the expression profiles of LePin and Granulin 1 in the 16 clusters
defined by scRNA-seq. Violin plots show the distribution of gene expression in
each of the 16 clusters. Cell numbers in cell clusters 1–16 are 2,794; 2,704; 2,073;
1,679; 1, 511; 1,374; 1,248; 1,069; 986; 923; 797; 649; 575; 321; 246 and 185,
respectively. b, Ultra-sensitive chromogenic RNA ISH by RNAscope probing for
Granulin 1 (left) and control (right). Positive signals are blue. Nuclei were
counterstained as purple with haematoxylin. White arrows indicate algae of
the Symbiodiniaceae. Six polyps from three independent experiments were
used for each probe. Scale bars, 10 μm. c, Percentage of alga-containing cells
with positive Granulin 1 or LePin signal. Each black dot stands for one section.
Red dots and lines stand for mean and s.d., respectively. Six polyps from three
independent experiments were used for one gene or control. d, Percentage of
alga-containing cells measured by FACS and microscopy. For FACS, each dot
stands for an individual polyp. Three independent experiments were
performed with each experiment, using two polyps. For microscopy, each dot
stands for a section analysed in three polyps. e, Heat map showing the
enrichment levels of the 89 marker genes in cluster 16 among all 16 cell clusters.
Transporters are highlighted in blue.