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Extended Data Fig. 6 | Additional analyses of endosymbiotic cell lineage.
a, A representative image of BrdU labelling (pink), overlaid with Hoechst (blue
DNA stain) in a cross-section of a regenerating Xenia sp. stalk. White, red and
green arrows indicate BrdU-negative (BrdU−) Xenia nuclei, an alga and a
BrdU-positive (BrdU+) Xenia nucleus juxtaposed to the alga, respectively. Three
regenerating stalks were used in two independent experiments. b, Box plot.
Percentages (y axis) of BrdU+ Xenia cells at the indicated regeneration time
points (x axis). Each dot represents data from one section. Three regenerating
stalks from two independent experiments were pooled and plotted for each
time point. About 7 to 18 sections were used for each group. The
alga-containing proliferated Xenia cells were estimated as those with BrdU+
nuclei juxtaposed to algae. The medians are indicated as lines in the box; the
upper and lower edge of the boxes represent the upper and lower quartiles,
respectively. c, Velocyto analysis of the scRNA-seq data from day-4
regenerating Xenia sp. stalks. Each dot represents a cell, and arrows indicate
the directions of RNA velocity. The green and red endosymbiotic cell clusters
were predicted as early and late-cell states, respectively, on the basis of the
directions of the arrows. d, The distribution of early (green) and late (red)
endosymbiotic cells predicted by velocyto in c on the pseudotime plot of all
scRNA-seq data shows the start and the direction of progression of the
endosymbiotic cell lineage.