Nature - USA (2020-06-25)

(Antfer) #1

Extended Data Fig. 4 | Calcium indicator is expressed exclusively in HVC
excitatory neurons and imaged in annotated ROIs. a, Sagittal slice of HVC
showing GCaMP-expressing PNs (experiment repeated in five birds with similar
re sult s). b, We observed no overlap between transduced GCaMP6f-expressing
neurons and neurons stained for the inhibitory neurons markers calretinin
(CR), calbindin, and parvalbumin (calretinin stain shown, staining experiment


repeated six times for each marker with similar results). c–e, Examples of daily
ROI annotation in three birds (1–3). Coloured circles mark different ROIs,
manually annotated on maximum f luorescence projection images on an
exemplary day (see Methods). f, Maximum f luorescence images (from bird 1;
see Methods) revealing f luorescence sources, including sparsely active cells, in
the imaging window across multiple days.
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