Nature - USA (2020-06-25)

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nature research | reporting summary

October 2018

Field-specific reporting

Please select the one below that is the best fit for your research. If you are not sure, read the appropriate sections before making your selection.

Life sciences Behavioural & social sciences Ecological, evolutionary & environmental sciences

For a reference copy of the document with all sections, see nature.com/documents/nr-reporting-summary-flat.pdf

Life sciences study design

All studies must disclose on these points even when the disclosure is negative.

Sample size The sample sizes are similar to sample sizes used in the field. No statistical methods were used to determine sample size.

Data exclusions We did not exclude any animal for data analysis. We excluded data from the very rare occasions in which video files were corrupted because

of tethering malfunctions.

Replication We performed recordings from multiple animals to confirm reproducibility. Replications were successful.

Randomization Our study did not include experimental groups and did not require randomization.

Blinding Our study did not include experimental groups and did not require blinding.

Reporting for specific materials, systems and methods

We require information from authors about some types of materials, experimental systems and methods used in many studies. Here, indicate whether each material,

system or method listed is relevant to your study. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response.

Materials & experimental systems

n/a Involved in the study

Antibodies

Eukaryotic cell lines

Palaeontology

Animals and other organisms

Human research participants

Clinical data

Methods

n/a Involved in the study

ChIP-seq

Flow cytometry

MRI-based neuroimaging

Antibodies

Antibodies used Immunohistochemistry:

Anti-Calbindin(SWANT CB38, rabbit, lot # 9.03, 1:4000), Anti-Calretinin(SWANT 7697, rabbit, lot # 1893-0114,1:15000), Anti-

Parvalbumin (SWANT PV27, rabbit, lot # 2014, 1:1000)

Validation All antibodies are used in a large number of publications and do not require additional validation.

References in manufacturer's website:

Anti-Calbindin:

Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493

Anti-Calretinin:

1. Schwaller B., Buchwald P., Blümcke I., Celio M.R. and Hunziker W. (1994)
   Characterization of a polyclonal antiserum against the purified human recombinant
   calcium-binding protein calretinin. Cell Calcium 14: 639-648.


2. Schiffmann S.N. et al (1999) Impaired motor coordination and Purkinje cell
   excitability in mice lacking calretinin. PNAS, 96: 5257-5262.


3. Gotzos V., Vogt P. and M.R. Celio (1995) Calretinin is a selective marker for
   malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192:137-


4. 
   


5. Doglioni, C. et al. (1996) Calretinin: a novel immunocytochemical marker for
   mesothelioma. Am. J. Surg. Pathol. 20:1037-1046.
   Anti-Parvalbumin:


6. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press


7. Celio M.R., Heizmann C.W. (1981) Nature 293: 300-302


8. Celio M.R., Heizmann C.W. (1982) Nature 297:504-506


9. Schwaller B., et al. (1999) Am. J. Physiol. 276. C395-403