Article
Extended Data Fig. 10 | shPTB-converted neurons replenish lost
dopaminergic neurons in substantia nigra. a, Schematic of the experimental
schedule for 6-OHDA-induced lesion followed by reprogramming with
A AV-PTB and then TH staining. b, c, Low-magnification views of unlesioned
substantia nigra stained for TH (b) and substantia nigra lesioned with 6-OHDA
and transduced with A AV-shPTB (c). Scale bars, 80 μm. These data were used to
provide the quantitative information shown in Fig. 4f, g. d, Enlarged view of
RFP+ cells that co-expressed TH in substantia nigra. Two RFP+TH+ cell bodies
are highlighted by orthogonal views of z-stacked images, attached on the right
and bottom of the main image in each panel. Scale bar, 10 μm. Results show the
generation of TH+ DA neurons in a highly region-specific manner in substantia
nigra, as a large population of RFP+ cells were not labelled by TH staining in the
same image. All experiments shown in this figure were independently repeated
3 times with similar results.