Extended Data Fig. 11 | Restoration of TH+ neurons in striatum of
6-OHDA-lesioned mice. a, b, Schematic of the coronal section of striatum and
images of uninjured control and lesioned striatum treated on the right side of
the brain with either A AV-empty or A AV-shPTB (a). Scale bar, 500 μm.
b, Magnified images showed extensive colocalization of TH with RFP-labelled
fibres. Scale bar, 10 μm. Results show a significant degree of restoration of TH+
fibres in striatum. Experiments were independently repeated 3 times with
similar results. c, d, Quantitative analysis of TH+ fibres in striatum under
different treatment conditions. TH staining of striatum under different
treatment conditions, as indicated (c). Scale bar, 10 μm. d, Quantification of
total TH+ or TH+RFP– fibre density in striatum under different treatment
conditions based on 3 biological repeats. Statistical significance was
determined by ANOVA with post hoc Tukey test; mean ± s.e.m. P-values are
indicated. Results show that most TH+ fibres seem to derive from
A AV-shPTB-converted dopaminergic neurons; however, the data do not rule
out the possibility that the axons of some endogenous neurons also responded
to the environment created by newly converted neurons.