Extended Data Fig. 13 | Measurement of striatal dopamine by HPLC and
controls with AAV-shGFP and AAV-hM4Di. a, b, Dopamine levels in brain
detected by HPLC with two different doses of spiked dopamine (a). b, Standard
curve generated from the spiked dopamine. This set of experiments was
performed only once. c, Controls for behavioural tests, showing that
expressing an anti-GFP control shRNA alone did not rescue chemical-induced
behavioural deficits based on apomorphine-induced rotation (left) and
cylinder test (right). d, Controls for behavioural tests, showing that the
expression of hM4Di in non-reprogrammed astrocytes did not trigger
detectable behaviour change in non-lesioned mice in the presence of CNO.
Statistical significance was determined by ANOVA (c, d); mean ± s.e.m. Six mice
were analysed in each group. P-values are indicated.
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