Article
Extended Data Fig. 14 | Electrophysiological analysis of PTB ASO-induced
neurons in vitro and in brain. a–c, Converted neurons showed large currents
from voltage-dependent sodium and potassium channels (a), repetitive action
potentials (b) and spontaneous postsynaptic currents (c). The numbers of
cells that showed the recorded activity versus the total number of cells
examined are indicated on the top right in each panel. d, Schematic of
transgenic mice used to trace astrocytes in vivo. e, Three weeks after tamoxifen
treatment, none of the tdTomato-labelled cells in the midbrain of Gfap-cre
ER:Rosa-tdTomato mice stained positive for NeuN (left), and all were GFAP+
(right). Scale bar, 50 μm. f–i, Converted neurons in brain slices showed large
currents from voltage-dependent sodium and potassium channels (f),
repetitive action potentials (g), spontaneous action potentials (h) and
spontaneous postsynaptic currents (i). The numbers of cells that showed the
recorded activity versus the total number of cells examined are indicated. The
results show that functional neurons are induced by PTB ASO both in culture
and in mouse brain. All experiments shown in this figure were independently
repeated twice with similar results.