Nature - USA (2020-06-25)

Article

1a

1b 7a

S E 6 N

kb 0102030

F5 F9

F4 F6 F12

F3

F2

F1

F10

F7

F8

F11

pT7-5’ UTR 3’ UTR-An

a

3a/bM

7b

8

3a/b

E

M

6

7a

GFP

7a

7b

8

N

F13

F14

F15

5A

5B

5C

5D

7Aa

7Ab

7B

J13 (1,069 bp)

J11 (900 bp)

J7 (664 bp)

J3 (530 bp)

J5 (382 bp)

J9 (772 bp)

J1 (235 bp)

500 bp

1,000 bp

200 bp

J12 (1,016 bp)

J8 (675 bp)

J6 (536 bp)

J4 (423 bp)

J10 (809 bp)

J2 (237 bp)

Junction

PCR-amplified

(amplicon size)

J14 (901 bp)

J1J2 J3 J4 J5 J6 J7 J8 J9 J10 J11 J12 J13

J14

Junction

PCR-amplified

(^12341234) (amplicon size)
Clones analyzed Clones analyzed
500 bp
1,000 bp
M
500 bp
1,000 bp
200 bp
M
J2 (1,157 bp)
J1 (842 bp) J5 (625 bp)
J3 (541 bp)
J4 (292 bp)
500 bp
1,000 bp
5A
5B
5C
5D
pCC1BAC-His3
J1
J2 J3 J4
J5
7Aa
7Ab
7B
pCC1BAC-His3
J6
J7 J8
J9
Junction
PCR-amplified
(amplicon size)
Clones analyzed
M 12345678910
b c
E
Junction
PCR-amplified
(^1234) (amplicon size)
Clones analyzed
M
J6 (1,059 bp)
J9 (849 bp)
J8 (549 bp)
J7 (198 bp)
500 bp
1,000 bp
Junction
PCR-amplified
(amplicon size)
Clones analyzed
M 123456
Extended Data Fig. 4 | See next page for caption.