Article
EIC (m/z= 389.2697)retention time (min)10 25
retention time (min)10 25retention time (min)10 25
retention time (min)10 25retention time (min)10 253-oxo-4,5-dehydro-
DCA ( 7 )3-oxo-4,5-6,7-
didehydro-DCA ( 6 )3-oxo-CA (3b) 3-oxo-DCA ( 8 )DCA ( 9 )EIC (m/z= 385.2384)EIC (m/z= 405.2647)EIC (m/z= 387.2541)EIC(m/z= 391.2854)standardexperimentally
observedstandardexperimentally
observedstandardexperimentally
observedstandardexperimentally
observedretention time (min)0cholyl-CoA ( 2 )EIC (m/z= 1158.4001)standardexperimentally
observedstandardexperimentally
observedretention time (min)5203-oxo-4,5-6,7-
didehydro-DCA ( 6 )EIC(m/z= 385.2384)standardexperimentally
observedbaEIC (m/z= 389.2697)retention time (min)2 15023-oxo-DCA ( 8 )standardexperimentally
observedExtended Data Fig. 3 | Bile acid standards. a, For each compound in the study
for which we have an authentic standard, we show an EIC of the authentic
standard and the experimentally observed compound. Because the data shown
here were collected from samples run at different times, a drift in retention
time may be responsible for the peak pairs that do not have identical retention
times. b, We observed a drift in retention time in the LC–MS data collected for
the experiment shown in Fig. 2c. For two representative compounds from that
data set, we show an EIC of the experimentally observed compound and an
authentic standard run contemporaneously, showing that the retention times
remain consistent with our peak assignments.