BaiCD BaiH
Km 59.7 +/- 13.2 M
Vmax 0.38 +/- 0.03 M/min
Kcat 0.84 +/- 0.06 min-1
Km 5.81 +/- 3.88 M
Vmax 2.53 +/- 0.42 M/min
Kcat 5.63 +/- 0.93 min-1
Extended Data Fig. 4 | Kinetic parameters for BaiCD and BaiH. a, Michaelis–
Menten analysis of the conversion of 3-oxo-4, 5-dehydro-DCA to 3-oxo-DCA by
BaiCD. Reaction mixtures contained 0.45 μM BaiCD and 1 mM NADH, with the
substrate concentration varying between 15 μM and 500 μM. b, Michaelis–
Menten analysis of the conversion of 3-oxo-4, 5,6,7-didehydro-DCA to 3-oxo-
4, 5-dehydro-DCA by BaiH. Reaction mixtures contained 0.45 μM BaiH and 1 mM
NADH, with the substrate concentration varying between 3 μM and 100 μM.
Data indicate the average product level ± 1 s.d. (three biological replicates).