retention time (min)
11 13 15 17 19 21
Cs + baiGCs + baiB-Iselected ion abundanc
e
m/z calc: 391.2854
m/z meas: 391.2853
CDCAm/z calc: 375.2905
m/z meas: 375.2909
LCAExtended Data Fig. 6 | 7α-dehydroxylation of CDCA in vivo. Combined EICs
showing the conversion of CDCA to LCA by a C. sporogenes strain harbouring
the complete bai operon on three plasmids (MF001) versus a control strain of C.
sporogenes harbouring the transporter baiG (MF012). The strains were
cultivated with 1 μM cholic acid for 72 h; an acetone extract of the culture
supernatant was analysed by high-performance LC (HPLC)/MS. The single
asterisk indicates isoLCA; the peak indicated by the double asterisk is
provisionally assigned as isoCDCA. This experiment was performed once.