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baiBspoIIE promoterbaiCDbaiEbaiA2baiFbaiGbaiHbaiIbai operonfdx promoterspoIIE promoterspoIIE promoterfdx promoterbaiGbaiHIbaiHbaiIfdx promoterpMF01 baiB-FpMF02pMF03pMF04 baiB-F ∆BpMF05 baiB-F ∆CDpMF06 baiB-F ∆EpMF07 baiB-F ∆A2pMF08 baiB-F ∆FpMF09 baiCDpMF10 baiHpMF1 1pMF12fdx promoterpMTL83153 pMTL83353 pMTL83253 repH
ermBtraJoriginrepHaad9traJoriginrepHcatPtraJoriginExtended Data Fig. 7 | Constructs for expressing the bai operon and
portions thereof in C. sporogenes. Each of the plasmids has replication
origins (origin and repH) for E. coli and Clostridium, the traJ gene to enable
conjugal plasmid transfer, and an antibiotic-resistance gene (catP, aad9 or
ermB). The bai genes were introduced into these plasmids under the control of
the fd x or spoIIE promoter. For the genetic analysis of baiCD and baiH function,
pMTL83153-based plasmids were used.