Nature - USA (2020-06-25)

Article

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Extended Data Fig. 3 | Topography rescues dendritic cell locomotion in the
absence of adhesion. a, Top left, scheme of a mature dendritic cell migrating
in a channel designed for the EDTA experiment. Mature dendritic cells migrate
in a device containing channels with different serration periods, with or
without 30 mM EDTA. Top right, single-cell migration speed with (+, blue) and
without (-, grey) EDTA treatment. Bottom, histograms of cell speed in different
serrations with (blue) or without (grey) EDTA. Representative of three
independent experiments. n = 35 cells without EDTA, n = 17 cells with EDTA.
Data are mean ± s.d.; P = 0.0022, **P < 0.0001 and ns P = 0.6363, one-way
ANOVA with Kruskal–Wallis test followed by post hoc Dunn’s test. b, Top left,

scheme of a mature dendritic cell migrating in a channel designed for the EDTA

experiment, along a CCL19 gradient (indicated in red). Mature dendritic cells

migrate towards the chemokine CCL19 into a device that contains

microchannels with different serration periods, with or without 30 mM EDTA.

Top right, migration speed with (+, blue) and without (-, grey) EDTA treatment.

Bottom, histograms of cell speed in the different serrated channels with (blue)

or without (grey) EDTA. Representative of three independent experiments.

Without EDTA: n = 44 cells; with EDTA: n = 52 (6–12 μm), n = 44 (24 μm), n = 33

(smooth channels). Data are mean ± s.d.; ***P = 0.0001 and ****P < 0.0001,

one-way ANOVA with Kruskal–Wallis test followed by post hoc Dunn’s test.