Nature - USA (2020-06-25)

Article

Agarose

ab

50μm

perpendicular

to ridges

parallel to ridges

side view

top view

800nm

600 nm

800nm

Agarose

WT and Lifeact-GFP

primary T cell

side view

top view

Agarose

WT and Lifeact-GFP

primary T cell

or

Agarose

ICAM-1

Agarose

Pll-PEG

c

d

X (μm)

Y (μm)

Cell velocity (μm/min

)

Smooth

PEG

30

20

10

0

Ridges

PEG

Smooth

ICAM-1

****

****

****

Ridges

PEG

Smooth

PEG

Smooth

ICAM-1

-600 -3000 300 600

-600

600

-300

0

300

e

f

-600 -300 0 300 600

-600

600

-300

0

300

-600 -300 0 300 600

-600

600

-300

0

300

Smooth

PEG

Mean instantaneou

s

cell velocity (μm/min)

30

20

10

0

Ridges

PEG

Smooth

ICAM-1

**** ns

****

90°

0°

-30°

-90°

-120°

-150°

60°

30°

120°

150°

180°

-60°

90°

0°

-30°

-90°

-120°

-150°

60°

30°

120°

150°

180°

-60°

90°

0°

-30°

-90°

-120°

-150°

60°

30°

120°

150°

180°

-60°

g

Pll-PEG

Extended Data Fig. 6 | See next page for caption.