Article
Extended Data Fig. 8 | The blue anthocyanidin and its potential biosynthesis
pathway in N. colorata. a, The peak of the blue anthocyanidin appears at 3 min
of the high-performance liquid chromatography (HPLC) detection.
b, The three fragments of the blue anthocyanidin and their molecule mass.
c, The molecule of the anthocyanidin was identified as delphinidin 3′-O- (2 ′′-O-
g a l l oy l- 6′′-O-acetyl-β-galactopyranoside), abbreviated as Dp3′galloyl-
acetylGal. d, The postulated pathway for the biosynthesis of Dp3′galloyl-
acetylGal. Gene copy numbers are listed next to the enzymes. 3GGT,
anthocyanidin 3-O-glucoside-2′′-O-glucosyltransferase; 3′GT, 3′-O-beta-
glucosyltranferase; 5AT, anthocyanin-5-aromatic acyltransferase; ANS,
anthocyanidin synthase; CHI, chalcone isomerase; CHS, chalcone synthase;
DFR, dihydrof lavonol-4-reductase; F3H, f lavanone-3-hydroxylase; F3′5′H,
f lavonoid-3′,5′-hydroxylase. e, Comparative transcriptomic analyses between
the blue- and white-petal cultivars of N. colorata identified two genes, ANS and
UDPGT, that are highly differentially expressed and might be potential
regulators for blue coloration of the petals.