Extended Data Fig. 1 | Expression of meristematic-zone marker and
transcriptome analysis upon RGF1 treatment. a, b, Confocal images of HPY2-
GFP roots 24 h after treatment with water (mock; a) or 20 nM RGF1 (b).
Seedlings were grown on MS plates for seven days before treatment. Left, PI-
stained roots; right, GFP signals. White and blue arrowheads indicate the
junction between the meristematic and elongation zones. Scale bar, 50 μm.
c, Area of HPY2-GFP expression (in μm^2 ; n = 8 independent roots; P < 2 .1 × 10−7).
Bar graphs show means. Error bars are ± s.d. Dots indicate each data point.
P values are calculated by two-sided Student’s t-test. d, Method of RNA
extraction following RGF1 treatment.