Nature - USA (2020-01-02)

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nature research | reporting summary

October 2018

Data

Policy information about availability of data

All manuscripts must include a data availability statement. This statement should provide the following information, where applicable:

• Accession codes, unique identifiers, or web links for publicly available datasets


• A list of figures that have associated raw data


• A description of any restrictions on data availability
  The original RNA sequencing data is uploaded and available online (Gene Expression Omnibus: GSE127572).

Field-specific reporting

Please select the one below that is the best fit for your research. If you are not sure, read the appropriate sections before making your selection.

Life sciences Behavioural & social sciences Ecological, evolutionary & environmental sciences

For a reference copy of the document with all sections, see nature.com/documents/nr-reporting-summary-flat.pdf

Life sciences study design

All studies must disclose on these points even when the disclosure is negative.

Sample size No sample size calculations were performed. For the in vitro experiments the variability between biological repeats was very low, when

possible at least 3 independent biological cell lines were analysed at least twice. For in vivo experiments, for each experiments at least 3 to 5

animals per genotype were used and experiments were performed twice to ensure reproducibility.

Data exclusions No data were excluded from the study.

Replication Experiments were reproduced at least twice and all attempts at replication were successful.

Randomization Mice were grouped according to genotype and animals were age- and sex-matched.

Blinding Animal technicians were blinded to treatment conditions and temparture and body temperature measurements without any input from the

experimental investigator.

Reporting for specific materials, systems and methods

We require information from authors about some types of materials, experimental systems and methods used in many studies. Here, indicate whether each material,

system or method listed is relevant to your study. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response.

Materials & experimental systems

n/a Involved in the study

Antibodies

Eukaryotic cell lines

Palaeontology

Animals and other organisms

Human research participants

Clinical data

Methods

n/a Involved in the study

ChIP-seq

Flow cytometry

MRI-based neuroimaging

Antibodies

Antibodies used RIPK1 N-terminal antibody (clone D94C12, cat number 3493, Cell Signaling Technology)

RIPK1 C-terminal antibody (cat number 610459, BD Transduction Laboratories)

Phospho-RIPK1 (clone D1L3S, cat number 65746, Cell Signaling Technology)

Phospho -RIPK3 (Gift from Genetech)

Caspase-6 (clone EPR4405, cat number ab108335, Abcam)

Caspase-8 (clone E7, cat number ab32397, Abcam)

Cleaved caspase-3 (cat number 9661, Cell Signaling Technology)

Cleaved caspase-8 (clone D5B2, cat number 8592, Cell Signaling Technology)

PECAM1 (cat number AF3628, R&D Systems)

goat anti-rabbit AF488 (cat number A-11008, Invitrogen)

donkey anti-goat cy3 (cat number 705-165-147, Jackson ImmunoResearch)

FADD (clone 7A2, WEHI in house)