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nature research | reporting summary
October 2018Instrument All events were acquired on a FACS Canto II cytometer.
Software All events were analyzed with FlowJo (10.0).
Cell population abundance 1.0E6 of PBMCs were used for each test. Coutess II FL (Thermo Fisher) and 0.4% Typan Blue were used to determine the viability
and number of cells. The viability of PBMCs is above 85% for each sample.
Gating strategy Surface markers CD3, CD4, CD14 and CD19 were used to gate total T cells, CD4+T cells, monocytes and total B cells respectively.^
A figure exemplifying the gating strategy is provided in the Supplementary Information.
Tick this box to confirm that a figure exemplifying the gating strategy is provided in the Supplementary Information.