Nature - USA (2020-01-02)

3

nature research | reporting summary

April 2018

Anti-Mouse CD31 (PECAM-1) eFlour 450, clone:390, Cat. #48-0311-82, LOT:1982691

ebioscience, 1:100, Flow

Anti-Mouse TER-119, VioletFluor450, clone:TER-119, Cat. #75-5921-U100, LOT:C5921081018753

Tonbo, 1:100, Flow

Anti-Mouse CD45, VioletFluor450, clone:30-F11, Cat. #75-0451-U100, LOT:C0451033117753

Tonbo, 1:100, Flow

Mouse Anti-Human HLA-ABC, FITC, clone:G46-2.6, Cat. #555552, LOT:8183993

BD Pharmingen, 1:20, Flow

Anti-Human MCT1, Alexa Fluor 647, clone:bs-10249R, Cat. #bs-10249R-A647, LOT:AE112116

Bioss antibodies, 1:100, Flow

Anti-Human CD147, Alexa Fluor 488, clone:HIM6, Cat. #306207, LOT:B213982, BioLegend, 1:100, Flow

Anti-Human CD98, PE-Vio 770, clone:REA387, Cat. #130-105-710, LOT:5170207052, Miltenyi Biotec, 1:200, Flow

Anti-Human beta 1-Integrin, Alexa Fluor 700, clone:P5D2, Cat. #FAB17781N-100UG, LOT:1529055, R&D systems, 1:100, Flow

Anti-Human CD324 (E-Cadherin), FITC, clone:67A4, Cat. #324104, LOT:B203125, BioLegend, 1:100, Flow

Anti-Human CD325 (N-Cadherin), PE/Cy7, clone:8C11, Cat. #350811, LOT:B272631, BioLegend, 1:100, Flow

Anti-Rabbit IgG, isotype control, Alexa Fluor 647, Cat. #bs-0295P-A647, LOT:AG0726809, Bioss antibodies,1:100, Flow

Anti-Mouse IgG1, k, isotype control, Alexa Fluor 488, clone:MOPC-21, Cat. #400129, LOT:B220820, BioLegend, 1:100, Flow

Anti-Human IgG1, isotype control, PE-Vio 770, clone:REA293, Cat. #130-113-452, LOT:5190329516, Miltenyi Biotec,1:100, Flow

anti-Mouse IgG1,k, isotype control, Alexa Fluor 700, clone:11711, Cat. #IC002N, LOT:ACIJ0418111, R&D systems, 1:100, Flow

Anti-MCT1, clone:HPA003324, Cat. #HPA003324-100UL, LOT:C75340, Sigma, 1:500, IF

Anti-Rabbit IgG, biotinylated, Cat. #PK-6104, Vector Laboratories, 1:250, IF

Anti-Rabbit IgG, peroxidase conjugated, Cat. #PK-6101, Vector Laboratories, 1:250, IF

Anti-S100, Cat. #Z0311, polyclonal, LOT:00060051, Dako, 1:500, IF

Donkey anti-Rabbit-IgG, Alexa Fluor 488 AffiniPure F(ab')2 fragment, Cat. #711-545-152, JacksonImmuno, 1:250, IF

Donkey anti-rat IgG, Cy3-AffiniPure F(ab')2 fragment, Cat. #712-166-150, JacksonImmuno, 1:250, IF

Anti-MCT1, Cat. #AB3538P, LOT:3190916, EMD Millipore, 1:5000, WB

Anti-MCT2, Cat. #LN2021159, LOT:5653586301013, LabNed, 1:5000, WB

Anti-MCT4, Cat. #AB3316P, LOT:2972442, EMD Millipore, 1:5000, WB

Ant-CD147, Cat. #ab64616, LOT:GR205454-1, Abcam, 1:10000, WB

Anti-Vimentin, clone:D21H3, Cat. #5741T, LOT:6 , Cell Signaling, 1:10000, WB

Anti-IKK-alpha, clone:D3W6N, Cat. #61294S, LOT:1, Cell Signaling, 1:5000, WB

Anti-IKK-beta, clone:D30C6, Cat. # 8943S, LOT:4, Cell Signaling, 1:5000, WB

Anti-LDHA, clone:C4B5, Cat. #3582 LOT:9, Cell Signaling, 1:10000, WB

Anti-LDHB, clone:EP1565Y, Cat. #ab53292, LOT:GR103088-10, Abcam, 1:10000, WB

Anti-alpha Tubulin, clone:EP1332Y, Cat. #ab52866, LOT:GR3241238-1, Abcam, 1:10000, WB

Anti-beta-Actin, clone:D6A8, Cat. #12620S, LOT:6, Cell Signaling, 1:10000, WB

Validation All antibodies are commercially available and have been validated in previously published studies (e.g. Nature 527:186).
Antibodies that were central to our conclusions, such as the anti-MCT1 antibodies, were validated with control lines (that were
positive or deficient for MCT1) and similar results were obtained using multiple independent antibodies.

Anti-Mouse CD31 (Cat. #48-0311-82, ebioscience). This monoclonal antibody recognizes mouse CD31. https://