Nature - USA (2020-01-02)

(Antfer) #1

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nature research | reporting summary


April 2018

http://www.thermofisher.com/antibody/product/CD31-PECAM-1-Antibody-clone-390-Monoclonal/48-0311-82


Anti-Mouse TER-119 (Cat. #75-5921-U100, Tonbo). This monoclonal antibody recognizes mouse TER-119. https://
http://www.tonbobio.com/violetfluortm-450-anti-mouse-ter-119-ter-119.html


Anti-Mouse CD45 (Cat.#75-0451-U100, Tonbo). This monoclonal antibody recognizes mouse CD45. https://www.tonbobio.com/
violetfluor-mouse-cd45-30-f11.html


Mouse Anti-Human HLA-ABC (Cat. #555552, BD Pharmingen). This monoclonal antibody binds to a monomorphic epitope on the
human alpha chain of HLA-A, HLA-B and HLA-C. http://www.bdbiosciences.com/us/applications/research/stem-cell-research/
mesenchymal-stem-cell-markers-adipose/human/positive-markers/fitc-mouse-anti-human-hla-abc-g46-26/p/555552


Anti-Human MCT1 (Cat. #bs-10249R-A647, Bioss antibodies). This polyclonal antibody recognizes extracellular epitopes of MCT1.
Species reactivity - Human, Mouse and Rat. We tested this antibody on MCT1-deficient cells (Fig. 2f) and found it to be specific
for MCT1. https://www.biossusa.com/products/bs-10249R-A647


Anti-Human CD147 (Cat. #306207, BioLegend). This monoclonal antibody recognizes human CD147. Species reactivity - Human.
https://www.biolegend.com/en-us/products/alexa-fluor-488-anti-human-cd147-antibody-3367


Anti-Human CD98 (Cat. #130-105-710, Miltenyi Biotec). This monoclonal antibody recognizes the human CD98 antigen. https://
http://www.miltenyibiotec.com/US-en/products/macs-flow-cytometry/antibodies/primary-antibodies/cd98-antibodies-human-
rea387-1-11.html?utm_source=3rd_labome%20&utm_medium=product_listing&utm_term=flow-
cytometry&utm_campaign=reafinity


Anti-Human beta 1-Integrin (Cat. #FAB17781N-100UG, R&D systems). This monoclonal antibody recognizes human Integrin beta
1/CD29. Species reactivity - Human. https://www.rndsystems.com/products/human-integrin-beta-1-cd29-alexa-fluor-700-
conjugated-antibody-p5d2_fab17781n


Anti-Human CD324 (E-Cadherin) (Cat. # 324104, BioLegend). This monoclonal antibody recognizes human CD324 also known as
E-cadherin. Species reactivity - human. https://www.biolegend.com/en-us/products/fitc-anti-human-cd324-e-cadherin-
antibody-3750


Anti-Human CD325 (N-Cadherin) (Cat. #350811, BioLegend). This monoclonal antibody recognizes human CD325, also known as
N-cadherin. Species reactivity - human. https://www.biolegend.com/en-us/products/pe-cy7-anti-human-cd325-n-cadherin-
antibody-9041


Rabbit IgG, isotype control (Cat. #bs-0295P-A647, Bioss antibodies). This polyclonal antibody is designed to serve as a control to
account for non-specific staining by primary antibodies that is caused by Fc Receptor-mediated binding or other isotype-specific
mechanisms. It was conjugated with Alexa Fluor 647. https://www.biossusa.com/products/bs-0295p-a647


Mouse IgG1k, isotype control (Cat. # 400129, BioLegend). This monoclonal antibody is designed to serve as a control to account
for non-specific staining by primary antibodies that is caused by Fc Receptor-mediated binding or other isotype-specific
mechanisms. It was conjugated with Alexa Fluor 488. https://www.biolegend.com/en-us/products/alexa-fluor-488-mouse-igg1--
kappa-isotype-ctrl-fc-2687


Human IgG1, isotype control (Cat. # 130-113-452, Miltenyi Biotec). This monoclonal antibody is designed to serve as a control to
account for non-specific staining by primary antibodies that is caused by Fc Receptor-mediated binding or other isotype-specific
mechanisms. It was conjugated with PE-Vio 770. https://www.miltenyibiotec.com/US-en/products/macs-flow-cytometry/
antibodies/isotype-control-antibodies/rea-control-antibodies-rea293-1-50.html


Mouse IgG1k, isotype control (Cat. # IC002N, R&D systems). This monoclonal antibody is designed to serve as a control to
account for non-specific staining by primary antibodies that is caused by Fc Receptor-mediated binding or other isotype-specific
mechanisms. It was conjugated to Alexa Fluor 700. https://www.rndsystems.com/products/mouse-igg-1-alexa-fluor-700-
conjugated-antibody_ic002n


Anti-human MCT1 (Cat. #HPA003324-100UL, Sigma). This polyclonal antibody recognizes human MCT1. This antibody has been
used previously to identify MCT1 in human cells (Cell Stem Cell. 20:635). This antibody was validated by the Human Protein Atlas
(HPA) project. https://www.proteinatlas.org/ENSG00000155380-SLC16A1.
https://www.sigmaaldrich.com/catalog/product/sigma/hpa003324?lang=en®ion=US


Anti-Rabbit IgG, biotinylated (Cat. #PK-6104, Vector Laboratories).
The biotinylated anti-rabbit IgG recognizes specifically rabbit IgG primary antibodies.
https://vectorlabs.com/vectastain-elite-abc-kit-rat-igg.html


Anti-Rabbit IgG, peroxidase conjugated (Cat. #PK-6101, Vector Laboratories).
The peroxidase anti-rabbit IgG recognizes specifically rabbit IgG primary antibodies. https://vectorlabs.com/vectastain-elite-abc-
kit-rabbit-igg.html


Anti-S100 (Cat. #Z0311, Dako). This polyclonal antibody strongly reacts with human S100B, and weakly or very weakly with
S100A1 and S100A6, respectively. This antibody has been used previously to identify S100 expression in melanoma cells (Nature
527:186). https://www.agilent.com/en/product/immunohistochemistry/antibodies-controls/primary-antibodies/s100-(dako-
omnis)-76198


Donkey anti-Rabbit-IgG, Alexa Fluor 488 AffiniPure F(ab')2 fragment (Cat. #711-545-152, JacksonImmuno). This polyclonal
antibody binds to rabbit IgG heavy and light chains. It also reacts with the light chains of other rabbit immunoglobulins. https://

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