Nature - USA (2020-01-02)

5

nature research | reporting summary

April 2018

http://www.jacksonimmuno.com/catalog/products/711-545-152

Donkey anti-rat IgG, Cy3-AffiniPure F(ab')2 fragment (Cat. #712-166-150, JacksonImmuno). This polyclonal antibody binds to

rabbit IgG heavy and light chains. It also reacts with the light chains of other rat immunoglobulins. https://

http://www.jacksonimmuno.com/catalog/products/712-166-150

Anti-MCT1 (Cat. #AB3538P, EMD Millipore).

This antibody recognizes human MCT1. This polyclonal antibody has been used previously to identify MCT1 expression in cancer

cells by western blotting (Cancer Res. 77:5591). We tested the specificity of this antibody by staining MCT1-deficient cells in Fig.

2a and ED Fig. 5a by western blot. http://www.emdmillipore.com/US/en/product/Anti-Monocarboxylate-Transporter-1-

Antibody,MM_NF-AB3538P

Anti-MCT2 (Cat. #LN2021159, LabNed). This polyclonal antibody recognizes epitopes of human MCT2. This antibody was tested

with a known positive control (MCF7 protein, Nat Chem Biol. 14:1032, Fig.1e) in Fig. 2b of this manuscript. https://labned.com/

slc16a7-human-unlb-antibody-ln2021159

Anti-MCT4 (Cat. #AB3316P, EMD Millipore). This polyclonal antibody recognizes human MCT4. This antibody has been used

previously to identify MCT4 staining in human cancer cells by western blotting (Cancer Res. 77:5591). We independently tested

the specificity of this antibody by staining positive and negative control cells in Fig. 2c and ED Fig. 5b of this manuscript by

western blot. https://www.emdmillipore.com/US/en/product/Anti-Monocarboxylate-Transporter-4-Antibody,MM_NF-AB3316P

Anti-CD147 (Cat. #ab64616, Abcam). This polyclonal antibody recognizes human and mouse CD147. The manufacturer validated

the antibody in western blots using synthetic peptides. https://www.abcam.com/cd147-antibody-ab64616.html

Anti-Vimentin (Cat. #5741T, Cell Signaling). This monoclonal antibody recognizes human, mouse, rat, and monkey vimentin

proteins. https://www.cellsignal.com/products/primary-antibodies/vimentin-d21h3-xp-rabbit-mab/5741

Anti-IKK-alpha (Cat. #61294S, Cell Signaling). This monoclonal antibody recognizes human, mouse, and rat IKKα proteins.

https://www.cellsignal.com/products/primary-antibodies/ikka-d3w6n-rabbit-mab/61294?site-search

type=Products&N=4294956287&Ntt=61294s&fromPage=plp&_requestid=3523507

Anti-IKK-beta(Cat. #8943S, Cell Signaling)..

This monoclonal antibody recognizes total IKKβ protein from human, mouse, rat, and monkey but does not cross-react with

other IKK family members. https://www.cellsignal.com/products/primary-antibodies/ikkb-d30c6-rabbit-mab/8943

Anti-LDHA (Cat. #3582, Cell Signaling). This monoclonal antibody recognizes endogenous levels of total LDHA protein. Species

Reactivity- Human, Monkey. https://www.cellsignal.com/products/primary-antibodies/ldha-c4b5-rabbit-mab/3582

Anti-LDHB (Cat. #ab53292, Abcam). This monoclonal antibody recognizes total human LDHB protein and the specificity was

validated by the manufacturer using LDHB-deficient HAP1 cells.

https://www.abcam.com/lactate-dehydrogenase-bldh-b-antibody-ep1565y-ab53292.html?productWallTab=ShowAll

Anti-Alpha Tubulin (Cat. #ab52866, Abcam). This monoclonal antibody recognizes mouse, rat, human, pig, and drosophila alpha

Tubulin. https://www.abcam.com/alpha-tubulin-antibody-ep1332y-microtubule-marker-ab52866.html

Anti-beta-Actin (Cat. #12620S, Cell Signaling). This monoclonal antibody

recognizes total β-actin protein in human, mouse, rat, monkey, drosophila, and zebrafish cells.

https://www.cellsignal.com/products/antibody-conjugates/b-actin-d6a8-rabbit-mab-hrp-conjugate/12620?site-search

type=Products&N=4294956287&Ntt=12620s&fromPage=plp&_requestid=3526589

Eukaryotic cell lines

Policy information about cell lines

Cell line source(s) YUMM1.7, YUMM3.3 and YUMM5.2 cell lines were purchased from ATCC.

Authentication YUMM1.7, YUMM3.3 and YUMM5.2 cell lines were authenticated by ATCC.

Mycoplasma contamination All cell lines were confirmed to be mycoplasma free by MycoAlert detection kit (Lonza).

Commonly misidentified lines

(See ICLAC register)

No commonly misidentified cell lines were used.

Animals and other organisms

Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research

Laboratory animals Four to 8-week-old NOD.CB17-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) and four to-8-week old C57/BL6 mice were used. Both male and

female mice were used.

Wild animals No wild animals were used.