to colleagues whose work we could not cite because of space
restrictions.Funding:Supported by NIH grant AI054636 and
the European Research Council (ERC Advanced Grant 268921 to
K.R.).Author contributions:J.S. and K.L.O. started the
experimental work at the Immune Disease Institute, Harvard
Medical School; R.G. substantially broadened and completed
the study at the MDC; K.-P.L. generated the VH12B1-8flimouse
strain; S.A. helped to generate the RNA-seq library; B.Z. helped
with cell transfer experiments; S.S. provided reagents; V.T.C.
provided scientific and experimental advice; R.G. and K.R.
wrote the manuscript; and K.R. supervised the project.
Competing interests:The authors declare no competing
financial interest.Data and materials availability:The RNA-
seq data are available in the Gene Expression Omnibus (GEO)
database (www.ncbi.nlm.nih.gov/geo) under accession number
GSE124827. The materials required to use the transgenic
system described here will be available through K.R. under a
material transfer agreement from the MDC.SUPPLEMENTARY MATERIALS
http://www.sciencemag.org/content/363/6428/748/suppl/DC1
Materials and Methods
Figs. S1 to S10
Table S1
References ( 35 – 40 )
22 July 2018; accepted 17 January 2019
10.1126/science.aau8475Grafet al.,Science 363 , 748–753 (2019) 15 February 2019 6of6
RESEARCH | REPORT
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