Chinet al.,Science 369 , 993–999 (2020) 21 August 2020 6of7
Fig. 4. Impact of systemic SR-717 administration on antitumor immunity
in mice.(A) Impact of systemic SR-717 delivery (administered as described
in Fig. 3C) on surface CD69 expression, assessed by flow cytometry, on
CD8 T cells isolated from tumors (TILs), spleens, or dLN of B16.F10 tumor-
bearing mice (n=4).(B) Impact of systemic SR-717 delivery (administered as
described in Fig. 3C) on surface CD69 expression, assessed by flow
cytometry, on NK cells isolated from tumors (TILs), spleens, or dLN of B16.
F10 tumor-bearing mice (n=4).(C) Impact of systemic SR-717 delivery
(administered as described in Fig. 3C) on granzyme B expression, assessed
by flow cytometry, on restimulated intratumoral and splenic CD8 T cells
(n=5).(D) Impact of systemic SR-717 delivery (administered as described
in Fig. 3C) on granzyme B expression, assessed by flow cytometry, on
restimulated intratumoral and splenic NK cells (n=5).(E)Impactof
systemic SR-717 (administered as described in Fig. 3C) on surface CD107a
expression on restimulated intratumoral and splenic CD8 T cells (n=5).
(F) Impact of systemic SR-717 (administered as described in Fig. 3C) on
surface CD107a expression on restimulated intratumoral and splenic NK cells
(n=5).(G) Impact of systemic SR-717 delivery (administered as described
in Fig. 3C) on surface expression of CD80 and CD86, determined by flow
cytometry, and on CD8a+DCs from dLN of B16.F10 tumor-bearing mice
(n=4).(H) Western blot analysis of the impact of SR-717 delivery (3.8mM)
on PD-L1 expression in WT THP1 or ISG-THP1 STING KO (STING KO) cells.
Vinculin was used as a loading control. (I) Impact of systemic SR-717 delivery
(administered as described in Fig. 3C) on PD-L1 surface expression on
CD8a−DCs from the dLN of B16.F10 tumor-bearing mice (n= 4). Data are
representative of three independent experiments, and values indicate mean ±
SEM with the exception of values in (I), which indicate mean ± SD. *P≤0.05;
**P≤0.01; ***P≤0.001; ****P≤0.0001; n.d., no difference.RESEARCH | REPORT