Article
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Acknowledgements We thank M. Schölling for help with statistics based on the ordinal
logistic regression model. We thank C. Kenyon for providing some C. elegans strains and
J. Fares for providing NP717. JM103 E. coli strains were provided by R. V. Aroian. Some
C. elegans strains and Microbacterium nematophilum were provided by the C. elegans
Genetics Center, which is funded by NIH Office of Research Infrastructure Programs
(P40 OD010440), the International C. elegans Gene Knockout Consortium and the National
BioResource Project (NBRP). This work was supported by funding from the DZNE (D.C.D.),
Max Planck Society (R.J.S.), the Deutsche Forschungsgemeinschaft (DFG, German Research
Foundation) (SFB 1035 to M. H., DA 1906/4-1 to D.C.D.) and a Marie Curie International
Reintegration Grant (322120 to D.C.D.).
Author contributions I.G., A.S, M.H., C.H. and D.C.D. designed the experiments and
interpreted data. I.G., A.S., R.J. and D.C.D. performed cloning and transgenic animal
generation. R.J., A.S. and D.C.D. performed insolubility analysis of LBP-2 and R.J. carried
out analysis of total LBP-2 levels with RNAi treatment. A.S. performed RNAi screen, LBP-2
aggregate counting and endocytosis evaluation. I.G. conducted LBP-2 aggregate counting,
lifespan and survival analysis. A.S and I.G. independently confirmed the effects of ECR
knockdown by RNAi on LBP-2 aggregation. J.L.B. and I.G. carried out aggregate counting
of LYS-7. I.G. and D.C.D. performed image acquisition and analysis. M.H. conducted
co-purification and western blots to detect interaction of LBP-2 and C36C5.5. I.G. collected
animals for RT–qPCR and S.A., R.J. and C.H. performed RT–qPCR. W.R. carried out RNA
sequencing. C.R. performed RNA-sequencing analysis. R.J.S. provided advice on RNA
sequencing. M.P. performed homology analysis and structure prediction. D.C.D. and I.G.
wrote the manuscript with contributions from R.J.S. and input from the other authors.Competing interests The authors declare no competing interests.Additional information
Supplementary information is available for this paper at https://doi.org/10.1038/s41586-020-
2461-z.
Correspondence and requests for materials should be addressed to D.C.D.
Peer review information Nature thanks Carmen Nussbaum and the other, anonymous,
reviewer(s) for their contribution to the peer review of this work.
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