Nature - USA (2020-08-20)

a

c

PbgA-prey

PbgA-bait LapB-bait

HisM-baitFtsH-bait

b

+ LapB + PlsY

E. coli K-12 ΔpbgA::pBADpbgA

+ LapB + PlsY

0.02% arabinose no arabinose

d

ef

LpxC

PbgAFLAG

GroEL

emptyPbgA WTPbgA T213DPbgA T213V

LpxC

LptD

GroEL

0.02 0.0020.0002

E. coli K-12

ΔlptD::pBADlptD

arabinose (%)

LpxC

GroEL

MsbA inhibitor

LapB-bait:

EptA_TMD-

PbgA_IFD+PD

WT

PbgA_TMD+IFD

PbgA_TMD-only

PbgA_M212A

PbgA_M212E

preys

PbgA_T213D

PbgA_T213V

PbgA_R216E

PbgA_R216A

PbgA_R215E

PbgA_R215A/

R216A

preys

E. coli K-12

ΔpbgA::pBADpbgA

no arabinose

E. coli K-12

ΔpbgA::pBADpbgA

no arabinose

pbgA

WT

pbgA

TMD-only

E. coli K-12

ΔpbgA::pBADpbgA

no arabinose

LpxC

GroEL

E. coli K-12

ΔpbgA::pBADpbgA

no arabinose

LpxC

PbgA

GroEL

pbgA

-FLAG

malEacpTacpS

hi

jk

g

pbgAWT

control pbgATM only

acpS acpT

Extended Data Fig. 8 | See next page for caption.