Mathewet al.,Science 369 , eabc8511 (2020) 4 September 2020 10 of 17
Fig. 5. Temporal relationships between immune responses and disease
manifestation.(A) Global viSNE projection of non-naïve CD8 T cells, non-naïve
CD4 T cells, and B cells for all participants pooled, with cells from COVID-19
patients at D0 and D7 concatenated and overlaid. Frequencies of (B) KI67+and
HLA-DR+CD38+CD4 T cells, (C) KI67+and HLA-DR+CD38+CD8 T cells, or
(D) PBs as indicated for HDs (green), RDs (blue), or COVID-19 patients (red),
with paired samples at D0 and D7 indicated by connecting lines. Significance
was determined by paired Wilcoxon test: *P< 0.05, **P< 0.01, ***P< 0.001,
and ****P< 0.0001. Longitudinal patterns (see Materials and methods) of
(E) HLA-DR+CD38+CD4 T cells or (F) PBs in COVID-19 patients shown as
frequency and representative flow cytometry plots. (G) Spearman correlations of
clinical parameters with longitudinal fold changes in immune populations.RESEARCH | RESEARCH ARTICLE