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ACKNOWLEDGMENTS
We thank G. Warren for comments on the manuscript. We thank
S. Brodesser and the CECAD Lipidomics/Metabolomics Facility for
performing lipidomics analyses. We thank M. Hartl from the Max
Perutz Labs Mass Spectrometry Facility, the Max Perutz Labs
BioOptics Facility, and the VBCF Electron Microscopy Facility for
technical support and the VBCF for providing the MS instrument
pool. Anti-CBP antibody and yeast strains carrying Atg2-Atg18
expression cassettes were provided by C. Ungermann. We thank
L. Pietrek for help with the simulation setup and D. Fracchiolla
for expressing and purifying unlabeled Atg21.Funding:This work
was supported by ERC grant 646653 (S.M.), Austrian Science
Fund FWF P32814-B (S.M.) and T724-B20 (J.S.-M.), Human
Frontier Science Program RGP0026/2017 (S.M., G.H., and S.v.B.),
an OEAW Doc fellowship (C.A.), and the Max Planck Society
(G.H., S.v.B., and M.G.).Author contributions:S.M., J.S.-M.,and G.H. designed and supervised research. V.B., N.C., S.v.B., and
V.N. designed research. J.S.-M., V.B., N.C., S.v.B., V.N., C.A.,
and M.S. performed research. All authors analyzed data and
commented on the manuscript. S.M. and J.S.-M. wrote the
manuscript.Competing interests:S.M. is a member of the
scientific advisory board of Casma Therapeutics.Data and
materials availability:All data are available in the manuscript
or the supplementary materials.SUPPLEMENTARY MATERIALS
science.sciencemag.org/content/369/6508/eaaz7714/suppl/DC1
Materials and Methods
Figs. S1 to S11
Tables S1 to S5
References ( 59 – 95 )
MDAR Reproducibility Checklist
Movie S1
Data S1
View/request a protocol for this paper fromBio-protocol.9 October 2019; resubmitted 16 May 2020
Accepted 6 July 2020
10.1126/science.aaz7714Sawa-Makarskaet al.,Science 369 , eaaz7714 (2020) 4 September 2020 10 of 10
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