Wanget al.,Science 369 , eaaz3090 (2020) 4 September 2020 4of9
Fig. 2. The RRP deployed by regeneration-specific cells is dysregulated in
regeneration-incompetent animals.(A) T-distributed stochastic neighbor
embedding (t-SNE) plot showing 13 different cell clusters identified in early KR;
7208 cells were included in the analyses. (B) t-SNE plot showing 16 different
cell clusters identified in early ZR; 8605 cells were included in the analyses.
(C) Annotation of killifish and zebrafish cell clusters. The expression offstl1in
the early killifish blastema cells was confirmed by in situ hybridization.
White dashed line indicates the amputation site. (D) Integrated single-cell
analysis between killifish and zebrafish. Left, annotation of major cell types.
Right, percentage of cells contributed by killifish and zebrafish. (E) Expression
of shared and species-specific blastema marker genes identified in the
integrated analysis. (F) Expression of 528 shared genes in different cell
types identified by scRNA-seq; 80 genes were specifically detected in the
blastema cells, and 232 genes were detected in two or more cell types.
(G) Examples of the expression of RRP genes in t-SNE–clustered killifish cells.
Only the enriched clusters are displayed for each gene. (H) Differential
regulation of 12 teleost-defined RRP genes between regenerating ear pinna in
the African spiny mouseA. cahirinus(blue) and nonregenerating ear pinna
in the house mouseM. musculus(green). Four representative genes are
highlighted in red.RESEARCH | RESEARCH ARTICLE