Nature - USA (2020-10-15)

a

CgSTING ΔTIR c CgSTING ΔTIR FsSTING ΔTM + 3',3'-cGAMP Human STING ΔTM + 2',3'-cGAMP

deee

R232

R151

FsSTING and Human STING Lids

f

P171

T173

P264 3'-OH

2'-OH

d

‘Open’ to ‘Closed’ transition

R151

R153

b

• 
  
  
  
  • 
    
  
  
  
  

2624319773_Flavobacteriaceae sp. genome_bin_

11

2523953452_Flavobacterium daejeonense DSM 17702654410348_Roseivirga ehrenbergii 2728687736_Flavobacteriaceae bacterium CRH

8

2568714955_T

enacibaculum maritimum NCIMB 2154

2804766972_T

enacibaculum maritimum NCIMB 2154

2790831227_T

enacibaculum agarivorans HZ1

2574465590_Arenibacter algicolaT

G409

2771484051_Winogradskyella arenosi CECT 79 2585882700_Psychroserpens sp. Hel_I_66 2587722571_Cellulophaga sp. Hel_I_12

(^58)
2790626827_Muricauda sp. SA2773508212_Leeuwenhoekiella sp. R48165
T19
644929227_Dyadobacter fermentans NS1
14_DSM 1805
3
2789740563_Confluentibacter sp. 3B2653570078_Mucilaginibacter gossypii Gh67
25722001
13_Sphingobacterium multivorum DSM 1
1691
2735805876_Sphingobacterium faecium DSM 1
169
0
2694664458_Chitinophaga sp
.YR627
2671579106_Mucilaginibacter pineti 47C3B
2819679791_Chitinophaga polysaccharea 12092743773958_Pedobacter panaciterrae O48
2628103216_Cyano_bin10_Bacteroidetes
2742562186_T
enacibaculum ovolyticum da5A8
2595697441_Larkinella arboricola DSM 21851 2710485422_Candidatus fluviicola riflensis RIFCSPHIGHO2_02Full43262709964249_Fluviicola sp. RIFCSPHIGHO2_01_FULL_43_53
0
2710805641_Fluviicola sp. RIFCSPHIGHO2_12_FULL_43_242784151568_Candidatus fluviicola riflensis RIFCSPHIGHO2_02Full4326
0
2802781326_Flavobacterium sp. SA
T1 3
0
2517580610_Parabacteroides sp.2587159478_Prevotella paludivivens JCM 13650 2515433181_Prevotella paludivivens DSM 17968
ASF519
2565659645_Bacteroides uniformis dnLKV2
2565662210_Bacteroides vulgatus dnLKV7
2565676688_Parabacteroides sp.
ASF519
2565698000_Parabacteroides goldsteinii dnLKV18 2807803812_Bacteroides caccae 2789STDY5834946 2600555872_Prevotella salivae F0493 2651290302_Porphyromonas sp. 54572_CO2648913936_Porphyromonas gulae COT052_OH1451
T. 108
2771437861_T
annerella sp. oral SAG Columbus 101
2800731 2682739943_Prevotella corporis MJR7716
183_Lachnospiraceae bacterium RUG226
2800306517_Prevotellaceae bacterium hRUG907 2578767730_Bacteroides fragilis J1434 2800976403_Prevotella sp. RUG047 2731877973_Dysgonomonas alginatilytica DSM 100214 26191 2719779365_Myroides sp. ZB35 2523953867_Flavobacterium daejeonense DSM 17708
10866_Chryseobacterium soli DSM 1929
8
2623152775_Chryseobacterium halperniae DSM 19921 2620728750_Chryseobacterium halperniae LMG 244012776772259_Chryseobacterium camelliae DolsongiHT1 2799567682_Lentisphaerae bacterium RUG67
3
2524412210_Pedobacter sp. V4
8
2597234634_Nonlabens sp. Hel_I_52596408080_Pedobacter luteus DSM 22385
6
2758613821_Maribacter cobaltidurans B1
2622875815_Algoriphagus ornithinivorans DSM 15282 2775577249_Leeuwenhoekiella nanhaiensis G18 2619920444_Chlorobi bacterium EBPR_Bin_19
0
2752156358_Unclassified Saprospiraceae HKUBAC3 2515843606_Balneola vulgaris DSM 17893
2718374542_Moheibacter sediminis CGMCC 1.12702586917340_Chryseobacterium hispalense DSM 25574
8
2623612695_Siphonobacter aquaeclarae DSM 2166
8
2620490221_Niabella drilacis DSM 2581 2619918617_Cytophagales bacterium EBPR_Bin_361
1
2625036409_Hymenobacter sp2685888458_Chryseobacterium frigidisoli DSM 26000
.AT0102
2629398417_Hymenobacter sp
.AT0102
2792180374_Arenibacter catalasegens P308H10 2700462282_Flavobacterium weaverense DSM 19727 2739647165_Pedobacter sp 2819413222_Pedobacter tournemirensis DSM 23085
.YR510
2790833744_T
enacibaculum agarivorans HZ
1
2530641 2686056644_Chryseobacterium soldanellicola DSM 17072 2799427151_Flavobacterium sp. 245
125_Aggregatibacter actinomycetemcomitans D17P3
2533353261_Aggregatibacter actinomycetemcomitans sv

. d I63B

2539262846_Aggregatibacter actinomycetemcomitans

Y4

2540567585_Aggregatibacter actinomycetemcomitans sv

. A D7S1

2682462656_Aggregatibacter actinomycetemcomitans sv

. e SA287

6

2688296686_Aggregatibacter actinomycetemcomitans 624 2731

143813_Aggregatibacter actinomycetemcomitans sv. d SA30

(^33)
2731
149251_Aggregatibacter actinomycetemcomitans sv. d SA22
(^00)
2541326748_Capnocytophaga granulos
aATCC 51502
2694988876_Capnocytophaga granulosa DSM 1144
9
649778979_Riemerella anatipestifer 2810445925_Capnocytophaga granulosa NCTC 12948
A350/72_DSM 15868
651
1461
13_Riemerella anatipestifer RAGD
2512834496_Riemerella anatipestifer
A350/72_DSM 1586
8
25341
17561_Riemerella anatipestifer RA
YM
2551889359_Riemerella anatipestifer RA
SG
2630660691_Riemerella anatipestifer
Yb2
2718681765_Riemerella anatipestifer RA15
3
2722955217_Riemerella anatipestifer RA17
2588554895_Riemerella anatipestifer CH3 2518774349_Riemerella anatipestifer RACH12641722961_Riemerella anatipestifer RAJL
LY
2521922030_Riemerella anatipestifer RACH2 2725874479_Riemerella anatipestifer RCAD0122
TM-STING
Extended Data Fig. 1 | See next page for caption.