Extended Data Fig. 1 | MeCP2 and HP1α are dynamic components of
heterochromatin condensates. a, Live-cell images of endogenous-tagged
HP1α–mCherry and Hoechst staining in mouse ES cells. b, Live-cell images of
endogenous-tagged MeCP2–GFP and HP1α–mCherry in mouse ES cells.
c, Live-cell images of FR AP experiments with endogenously tagged HP1α–
mCherry mouse ES cells. d, FR AP curves for experiments in c. Photobleaching
occurs at t = 0 s. n = 7 cells. e, Half-time of photobleaching recovery for
MeCP2–GFP and HP1α–mCherry at heterochromatin condensates in imaging
experiments in c and Fig. 1b. n = 7 cells per condition. P = 0.90, t = 0.13, df = 12,
two-tailed Student’s t-test. f, Mobile fractions of MeCP2–GFP and HP1α–
mCherry within heterochromatin condensates in imaging experiments in c and
Fig. 1b, determined by FR AP analysis. n = 7 cells per condition. P = 0.09, t = 1 .87,
df = 12, two-tailed Student’s t-test. Data are mean ± s.e.m.
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