Nature - USA (2020-10-15)

0.0

0.2

0.4

0.6

0.8

Mean diff

erenc

e

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

1.0

Choice Index

alg-1(gk214)

**** ****

N2 alg-1(gk214)

PAOP50 plateN214 plate alg-1-

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

1.0

drh-1(ok3495)

Choice Index

**** ****

N2 drh-1(ok3495)

0.0

0.2

0.4

0.6

0.8

Mean di

fferenc

e

OP50 platePAN214 plate drh-1-

-0.8

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

1.0

Choice Index

sid-2(qt42)

**** ****

N2 sid-2(qt42)

0.0

0.2

0.4

0.6

0.8

Mean dif

ferenc

e

PAOP50 plateN214 plate -sid-2

n.s.

24 h OP50 bacteria lawn24 h PA14 bacteria lawn

n.s.

24 h OP50 bacteria lawn24 h PA14 bacteria lawn

n.s.

24 h OP50 bacteria lawn24 h PA14 bacteria lawn

-0.8

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

1.0

dcr-1(mg375)

Choice Inde

x

**** ****

N2 dcr-1(mg375)

0.0

0.2

0.4

0.6

0.8

Mean dif

ferenc

e

PAOP50 plateN214 plate dcr-1-

24 h OP50 bacteria lawn24 h PA14 bacteria lawn

n.s.

-1.0

-0.8

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

1.0

rde-1(ne219)

Choice Inde

x

N2 rde-1(ne219)

**** ****

n.s.

PAOP50 plateN214 plate rde-1-

24 h OP50 bacteria lawn24 h PA14 bacteria lawn

24 h OP50 small RN24 h PA14 small RNAA

**

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

Choice Inde

x

**** n.s.

rde-1(ne219)

N2 rde-1(ne219)

0.0

0.2

0.4

0.6

0.8

Mean dif

ferenc

e

−0.2

0.0

0.2

0.4

0.6

Mean di

fference

PAOP50 sRNAN214 sRNArde-1 -

-1.0

-0.8

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

1.0

rde-2(pk1657)

Choice Inde

x

N2 rde-2(pk1657)

**** ***

0.0

0.2

0.4

0.6

0.8

Mean dif

ferenc

e

PAOP50 plateN214 plate rde-2-

n.s.

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

Choice Index

rde-2(pk1657)

**** n.s.

N2 rde-2(pk1657)

**

−0.2

0.0

0.2

0.4

0.6

Mean di

fference

PAOP50 sRNAN214 sRNA rde-2 -

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

1.0

Choice Inde

x

rde-4(ne305)

**** ****

N2 rde-4(ne305)

n.s.

24 h OP50 bacteria lawn24 h PA14 bacteria lawn

0.0

0.2

0.4

0.6

0.8

Mean di

fference

PAOP50 plateN214 plate rde-4-

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

1.0

mut-7(pk720)

Choice Index

**** ****

N2 mut-7(pk720)

24 h OP50 bacteria lawn24 h PA14 bacteria lawn

n.s.

0.0

0.2

0.4

0.6

0.8

Mean dif

ferenc

e

PAOP50 platN214 plate mut-e^7 -

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

Choice Inde

x

mut-7(pk720)

N2 mut-7(pk720)

**** n.s.

*

−0.4

−0.2

0.0

0.2

0.4

0.6

0.8

Mean di

fference

PA14 sRNA -

OP50 sRNAN2 mut-7

24 h OP50 small RN24 h PA14 small RNAA

a b

f

g

j l

m

k

d 24 h OP50 bacteria lawn

24 h PA14 bacteria lawn

OP50 plate PA14 plate-

N2dcr-1dcr-1;

int::dcr-1

0.0

0.2

0.4

0.6

0.8

1.0

Mean dif

ferenc

e

-0.8

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

1.0

Choice Inde

x

**** **** ****

N2dcr-1(mg375)dcr-1(mg375)intestine::dcr-1;

n.s.

n.s.

dcr-1intestinal rescue e

-0.8

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

1.0

Choice Index

**** ****

****

N2 sid-1(pk3321)

Mean dif

ferenc

e

PAOP50 plateN214 plate -sid-1

0.0

0.2

0.4

0.6

24 h OP50 bacteria lawnsid-1(pk3321)

24 h PA14 bacteria lawn

24 h OP50 bacteria lawn24 h PA14 bacteria lawn (^) 24 h OP50 small RN24 h PA14 small RNAA
-0.6
-0.4
-0.2
0.0
0.2
0.4
0.6
0.8
1.0
Choice Index
N2 alg-1(gk214)

---

n.s.
-0.6
-0.4
-0.2
0.0
0.2
0.4
0.6
0.8
Choice Index

---

N2 drh-1(ok3495)
n.s.
n.s.
-0.8
-0.6
-0.4
-0.2
0.0
0.2
0.4
0.6
0.8
Choice Inde
x
N2 dcr- 1(mg375)
**** n.s.
24 h OP50 small RN24 h PA14 small RNAA
c dcr-1(mg375)
alg-1(gk214)
24 h OP50 small RN24 h PA14 small RNAA
drh-1(ok3495)
24 h OP50 small RN24 h PA14 small RNAA
−0.2
0.0
0.2
0.4
0.6
0.8
Mean dif
ferenc
e
PAOP50 sRNAN214 sRNA-dcr-1
0.0
0.2
0.4
0.6
0.8
Mean di
fference
PAOP50 sRNAN214 sRNA-alg-1
0.0
0.2
0.4
0.6
0.8
Mean di
fference
OP50 sRNAPAN214 sRNA drh-1 -
h i
n o p
Extended Data Fig 2 | See next page for caption.