Nature - USA (2020-10-15)

Article

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Choice Inde

x

N2 dcr-1(mg375)

**** n.s.

P0 24 h OP50 small RNA

P0 24 h PA14 small RNA

n.s.

P0 24 h OP50 small RNA

P0 24 h PA14 small RNA

P0 24 h OP50 small RNA

P0 24 h PA14 small RNA

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Choice Inde

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**** n.s.

N2 sid-1(pk3321)

n.s.

F1 sid-1(pk3321)

P0 24 h OP50 bacteria lawn

P0 24 h PA14 bacteria lawn

F1 sid-2(qt42)

P0 24 h OP50 bacteria lawn

P0 24 h PA14 bacteria lawn

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Choice Index

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N2 dcr-1(mg375)

F1dcr-1(mg375)

P0 24 h OP50 bacteria lawn

P0 24 h PA14 bacteria lawn

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Choice Index

N2 sid-1(pk3321)

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**

F1 sid-1(pk3321)

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Choice Inde

x

N2 sid-2(qt42)

**** n.s.

n.s.

n.s.

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Mean di

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Mean di

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Mean dif

ference

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Mean di

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Mean di

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PA14 plate -

OP50 plate

N2 sid-1

PA14 plate -

OP50 plate

N2sid-2

PAOP50 plate14 plate -

N2dcr-1

PAOP50 sRNA14 sRNA -

N2 sid-1

PAOP50 sRNA14 sRNA -

N2 sid-2

PA14 sRNA -

OP50 sRNA

N2 dcr-1

a

b

c

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Choice Inde

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F1sid-2(qt42)

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n.s.

N2 sid-2(qt42)

F1dcr-1(mg375)

Extended Data Fig. 4 | s id-1, s i d-2 and dc r-1 F 1 progeny of plate and sRNA
trained parent worms. a–c, Progeny of sid-1 (a), s id-2 (b) and dcr-1 (c) mutants
are defective in both transgenerational pathogen avoidance following
maternal bacterial lawn (left) and PA14 sRNA training (right). Biological
replicates: 3 (a–c). For choice assays, each dot represents an individual choice
assay plate (average of 115 worms per plate) with data shown from all replicates.
For box plots, centre line is the median, box extends from the 25th to the 75th

percentile; whiskers denote minimum–maximum values. Two-way ANOVA

(a–c), Tukey’s multiple comparison test. **P ≤ 0.01, ***P ≤ 0.001, ****P < 0.0001,

n.s., not significant. Mean differences are shown using Cumming estimation

plots^47 , with each graphed as a bootstrap sampling distribution. Mean

differences are depicted as dots; 95% confidence intervals are indicated by the

ends of the vertical bars. See Supplementary Table 4 for exact sample sizes (n)

and P values.