Nature - USA (2020-10-15)

P11 17nt match

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

1.0

Choice Inde

x

P0 vhp-1(sa366)

**** ****

n.s.

N2 vhp-1(sa366)

-0.8

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

1.0

Choice Index

P0 vhp-1(sa366)

**** *

N2 vhp-1(sa366)

n.s.

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

-0.6

Choice Index

F1 vhp-1(sa366)

**** ***

n.s.

N2 vhp-1(sa366)

n.s.

a

0.0

0.2

0.4

0.6

Mean di

fference

PAOP50 plate14 plate -

N2vhp-1

0.0

0.2

0.4

0.6

Mean di

fference

OP50 sRNAPAN214 sRNAvhp-1 -

0.0

0.2

0.4

0.6

Mean di

fference

PAOP50 plateN214 plate -vhp-1

n.s.

n.s.

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

Choice Index

** n.s.

**

F1maco-1(ok3165)

N2 maco-1(ok3165)

n.s.

−0.4

−0.2

0.0

0.2

0.4

0.6

Mean di

fference

P11 sRNA

metabolites

innate immunity

Control

non-pathogenic

food

(pyochelin/PCN)

(pmk-1 dependent,

pmk-1 independent/irg-1)

maco-1

inactive pathway

food

Wild-type worms on OP50

PA14

attraction

Pseudomonas

exposure

active pathway

active pathway

Wild-type worms on PA14

PA14

avoidance

Control

non-pathogenic

food

active pathway

inactive pathway

food

maco-1 mutants on OP5 0

maco-1 mutants on PA 14

Pseudomonas

exposure

active pathway

active pathway

PA14

avoidance

inactive pathway

PA14

avoidance

P0 24 h OP50 small RNA P0 24 h PA14 small RNA 24 h OP50 bacteria lawn 24 h PA14 bacteria lawn

24 h OP50 small RNA 24 h PA14 small RNA P0 24 h OP50 bacteria lawn P0 24 h PA14 bacteria lawn

b

c d e

fg h

PA14 sRNA -

OP50 sRNA

N2maco-1

P11 sRNA

metabolites

(pyochelin/PCN)

maco-1

P11 sRNA

metabolites

innate immunity

(pyochelin/PCN)

(pmk-1 dependent,

pmk-1 independent/irg-1)

maco-1

innate immunity(pmk-1 dependent,

pmk-1 independent/irg-1)

P11 sRNA

metabolites

innate immunity

(pyochelin/PCN)

(pmk-1 dependent,

pmk-1 independent/irg-1)

maco-1

OP50

lawn

PA 14

lawn

0

1000

2000

3000

4000

5000

P0

normalized count

s

padj = 1. 13 x 10 -^26

F1

vhp-1 expression

OP50

lawn

PA 14

lawn

0

1000

2000

3000

4000

5000

normalized count

s

padj = 0. 16

-0.8

-0.6

-0.4

-0.2

0.0

0.2

0.4

0.6

0.8

Choice Index

N2 maco-1(ok3165)

* n.s.

***

F1maco-1(ok3165)

n.s.

−0.2

0.0

0.2

0.4

0.6

Mean di

fference

P0 24 h OP50 bacteria lawn P0 24 h PA14 bacteria lawn

OP50 platePAN214 plate maco-1-

ASI daf-7p::GFP

ASJ daf-7p::GFP

daf-7

independent

ASI daf-7p::GFP

ASJ daf-7p::GFP

daf-7

independent

ASI daf-7p::GFP

ASJ daf-7p::GFP

daf-7

independent

ASI daf-7p::GFP

ASJ daf-7p::GFP

daf-7

independent

Extended Data Fig. 7 | See next page for caption.