Article
Extended Data Fig. 7 | P11 region of homology with mac o -1 and behaviour of
vhp -1 mutants. a, Wormbase depiction of the maco-1 genomic locus and the
region of P11 homology (red). b, Model depicting induced learning pathways in
wild-type or maco-1 mutant worms as a result of OP50 or PA14 lawn exposure.
c, F 1 progeny of OP50 or PA14 sRNA-exposed maco-1 mutant mothers were
tested for PA14 avoidance behaviour. d, vhp-1expression is increased in PA14-
exposed mothers, but not in F 1 progeny^3. Mean; DESeq2 adjusted P values are
shown. P 0 , n = 6 replicates per condition; F 1 , n = 4 replicates per condition.
e, The vhp-1(sa 3 3 6) mutation is a null allele (early stop codon)^40. Wild-type and
vhp-1(sa 3 66) worms were trained on OP50 or PA14 bacterial lawns for 24 h and
tested for learned PA14 avoidance (top). f, Wild-type and vhp-1(sa 3 66) worms
were trained on OP50 or PA14 sRNA for 24 h and tested for learned PA14
avoidance. g, Progeny of vhp-1(sa 3 66) PA14 lawn-trained worms inherit PA14
avoidance behaviour. h, F 1 progeny of OP50 or PA14 lawn-exposed maco-1
mutant mothers were tested for PA14 avoidance behaviour. Biological
replicates: 3 (c, e–h). For choice assays, each dot represents an individual
choice assay plate (average of 115 worms per plate) with data shown from all
replicates. For box plots, centre line is the median, box extends from the 25th to
the 75th percentile; whiskers denote minimum–maximum values. Two-way
A N OVA (c, e–h), Tukey’s multiple comparison test. *P ≤ 0.05, **P ≤ 0.01,
***P ≤ 0.001, ****P < 0.0001, n.s., not significant. Mean differences are shown
using Cumming estimation plots^47 , with each graphed as a bootstrap sampling
distribution. Mean differences are depicted as dots; 95% confidence intervals
are indicated by the ends of the vertical bars. See Supplementary Table 4 for
exact sample sizes (n) and P values.