Extended Data Fig. 2 | Cryo-EM data collection and processing.
a, Representative micrograph (graphene oxide coated grid, imaged using a
Talos Arctica and a Gatan K3 camera) showing CUL1-SKP1-FBXL17-KEAP1V98A
particles. b, Resolution estimation using the FSC = 0.143 criterion^50 indicates
an overall resolution of 8.5 Å for the cryo-EM reconstruction. c. Data
processing scheme. Datasets were initially processed independently and then
combined for the final refinement. EM volumes are shown in grey and
orientation distributions are given for intermediate refinement steps. The final
reconstruction is shown with and without sharpening applied, and additionally
colored by local resolution (determined using RELION3). d, e, Initial model
generation. We originally obtained an initial reference by generating a
low-resolution volume with the overall shape of the complex observed in 2D
class averages (d) and later also verified this solution using CRYOSPARC2 ab
initio model generation (e; see Methods for details).