Article
Extended Data Fig. 11 | Phenotypes of larvae injected with 22 different
negative control sgRNAs. Embryos injected with an untargeted negative
control sgRNA + Cas9 had normal morphology at st. T30. Embryos injected
with 21 different sgRNAs targeting other developmental regulators expressed
in the larval lamprey head resulted in a range of different phenotypes at st.
T30 and st. T26.5. Heart oedema was not a feature of the mutant phenotype of
most sgRNAs. See figure for larvae numbers. These results confirm that the
phenotypes described by this work are not non-specific side effects of the
CRISPR /Cas9 method in the sea lamprey.