Nature - USA (2020-09-24)

(Antfer) #1

Extended Data Fig. 8 | Flg22-induced calcium inf lux measured in leaf discs
is comparable between wild-type and osca1.3/1.7 plants. a, Calcium inf lux in
leaf discs taken of Col-0 and osca1.3/1.7 plants expressing the calcium reporter
aequorin. f lg22 was added at time point 10 min. Error bars represent
mean ± s.d. (n = 12 leaf discs from 6 independent plants). The experiment was
performed twice with similar results. b, Average values of FRET ratio changes in
leaf discs of Col-0 and osca1.3/1.7 expressing the ratiometric calcium reporter
YC3.6 obtained in plate reader-based assays. Error bars show s.e.m., n = 90 leaf
discs (Col-0) and 47 leaf discs (osca1.3/1.7), with 6 leaf discs taken per individual
plant. The experiment was performed twice with similar results.

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