Article
Extended Data Fig. 5 | Canonical markers from the various intestinal cell
types are accurately reproduced in vitro. Heat map of unbiased top markers
(Methods) associated with the various cell type clusters found in vitro.
Canonical signature genes of stem cells (Olmf4, Lgr5, Axin2) form a continuous
gradient towards enterocytes (Fabp1, Apoa1) and villus-top enterocytes (Ada,
Krt20). Paneth cells express an array of antibacterial markers (defensins,
lysozyme), enteroendocrine cells are marked by chromogranins (Chga, Chgb)
and subpopulation-specific hormones. Tuft cells selectively express the
landmark gene Dclk1, as well as eicosanoid biosynthesis pathway enzymes
(Alox5ap, Hpgds, Ltc4s) and receptors implicated in taste transduction (Tr p m 5,
Gng13).