Extended Data Fig. 3 | Anatomy of the hepatic vagus nerve in mice.
a, Anatomical diagram. b, Operative field for hepatic vagotomy. c, A schematic
view showing the ignition of liver-brain-gut neural arc during colitis. d, The
common hepatic branch of the vagus does not contain sympathetic nerve.
Operative field for electrical recording of hepatic sympathetic nerve (left).
Electrical activity in common hepatic branch of the vagus and hepatic
sympathetic nerve, respectively (middle panel). Representative images of
immunof luorescence staining for Tyrosine hydroxylase (TH) in hepatic branch
and DRG (right panel, bar: 100 μm). e, Fluorescent immunohistochemistry of
TRPV1+ neuron in hepatic vagal branch at day 2 after capsaicin application (bar:
200 μm). f–h, WGA retrograde tracing. f, g, WT mice were subjected to Sham or
HVx and then were injected with Alexa Fluor 488 conjugated WGA at day 2 after
surgery (n = 3/group). Fluorescence image of Alexa Fluor 488+ neuron (green)
and DAPI (blue) in NG (f) and Th4 DRG (g) at 1 week after injection of WGA in
liver. Representative images (left panel, bar: 50 μm). Number of WGA+ neurons
(right panel). h, Fluorescence images of Alexa Fluor 488+ neuron (green) and
DAPI (blue) in DRG (Th4-7 and Th13) at 1 week after injection of WGA in liver
(bar: 100μm). Representative of two independent experiments (d–h). P values
obtained via unpaired two-tailed Student’s t tests. Data are shown as
mean ± SEM (f, g).