Extended Data Fig. 4 | Effects of vagotomy on maintenance and stability of
colonic pTreg. WT mice were subjected to Sham, VGx or HVx (n = 9/group).
Phenotypes and gene expression of colonic immune cell were analysed at day 2
after surgery. a, Frequency of Foxp3+ cells among CD4+ cells in colon.
Representative contour plots. b, Frequency of RORγt+ cells among Foxp3+ Tr e g
in colonic LP. Representative contour plots (left panel). Quantification (right
panel). c, Expression of Aldh1a1 and Aldh1a 2 mRNA in colonic APC.
d, Frequency of ALDH+ cells among MHC-II+ colonic APCs. Histograms of ALDH+
cells with colonic APCs (left panel). Quantification (right panel). e, f, WT mice
were subjected to Sham or HVx. Colonic T cell phenotypes were analysed at the
indicated time point following HVx (n = 9/group). Frequency of Foxp3+ cells
among CD4+ T cells in colonic LP (e) and RORγt+ cells among Foxp3+ Treg in
colonic LP (f). Representative contour plots (left panel) and quantification
(right panel) are shown (e, f). g–i, Frequency of Foxp3+ cells among CD4+ cells
(g–i) and RORγt+ cells among colonic Foxp3+ Tr e g (g, h) in colon at day 2 after
sur ger y. g, B6 mice were subjected to Sham or HVx (n = 10/group). h, BALB/c
mice were subjected to Sham or HVx (n = 4/group). i, Six-week-old WT rat were
subjected to Sham or HVx (n = 4/group). j, k, Rag2−/− mice were subjected to
sham-operation or HVx and then transferred with CD4+ CD45RBhi T cells (n = 8/
group). Four weeks after transfer, mice were sacrificed and colonic Treg cells
were analysed. j, Frequency of Foxp3+ cells among CD4+ cells in colon.
Representative contour plots (left panel). Quantification (right panel).
k, Frequency of RORγt+ cells among colonic Foxp3+ Treg. Representative
contour plots (left panel). Quantification (right panel). l, WT mice were
subjected to Sham or HVx. Colonic immune cell phenotypes were analysed 2
days later. Frequency of IFN-γ+, Gata3+ a n d I L-1 7A+ cells among CD4+ cells in
colon (n = 9/group). Representative of two (e, f, h, i) independent experiments
or pooled from three independent experiments (a–d, g, j–l). P values obtained
via one-way ANOVA with Tukey’s post hoc test (b–f) and unpaired two-tailed
Student’s t tests (g–l). Data are shown as mean ± s.e.m.