Article
Extended Data Fig. 7 | Effects of VGx and HVx on intrinsic enteric neuron.
a–c, g, WT mice were subjected to Sham (n = 4) or VGx (n = 5). d–f, h, WT mice
were subjected to Sham (n = 6) or HVx (n = 6). Activity of intrinsic enteric
neuron were measured at day 2 after surgery. a, d, Representative images of
immunof luorescence staining for HuC/D (white) and c-Fos (red) in colon. Scale
bar indicates 100 μm. b, e, Quantification of c-Fos+ neurons. c, f, Expression of
Hand2 mRNA in colon. g, h, Colonic acetylcholine, norepinephrine and CGRP
levels. * and ** indicate P < 0.05 and P < 0.01, respectively. P values obtained via
unpaired two-tailed Student’s t tests. Data are shown as mean ± s.e.m.