Extended Data Fig. 8 | Effects of mAChR and α7nAChR on maintenance of
colonic Treg. a-g, WT mice were subjected to Sham or HVx and then injected
daily with water or bethanechol (BETH; i.p. 300 μg/day) (a–d) or GST-21
(α7-agonist, i.p. 300 μg/day) (e–g) for 2 days (a, b, n = 5/group; c, d, n = 10/
group; e-g, n = 6/group). h–j, WT mice were subjected to Sham or HVx and then
daily injected with water, BETH only or BETH plus MLA (α7-antagonist) for 2
days (n = 6/group). k, l, WT and mAchR TKO mice were subjected to Sham or
HVx and then daily injected with water or BETH for 2 days (n = 4/group) related
to Fig. 3e. Phenotypes of colonic immune cell were analysed at 12 h after last
injection. a, g, j, l, Frequency of ALDH+ cells among MHC-II+ colonic APCs.
b, Expression of Aldh1a1 and Aldh1a 2 mRNA in colonic APC. c, e, h, Frequency
of Foxp3+ cells among CD4+ cells in colon. d, f, i, k, Frequency of RORγt+ cells
among colonic Foxp3+ Tregs. P values obtained via one-way ANOVA with
Tukey’s post hoc. Data are shown as mean ± s.e.m.