Article
Extended Data Fig. 7 | Activation of RagA is essential for mTOR lysosomal
recruitment and TFEB cytosolic localization. a, b, Representative
immunof luorescence images of endogenous mTOR (a) and endogenous TFEB
(b) upon transfection of a construct encoding active (RagA(Q66L)) or inactive
(RagA(T21L)) HA-tagged RagA or an empty vector in RagA-knockout HeLa cells.
Cells were deprived of amino acids for 50 min and then stimulated with amino
acids for 15 min. Scale bars, 10 μm.